Bioremediation through Rhizosphere Technology by Todd A. Anderson, Joel R. Coats

By Todd A. Anderson, Joel R. Coats

The 1st compilation of analysis on microbial degradation of contaminants within the rhizosphere. Discusses the interactions among microorganisms, crops, and chemical substances within the rhizosphere. Examines the degradation of business chemical compounds, together with polycyclic fragrant hydrocarbons, pentachlorophenol, and trichloroethylene, in addition to the degradation of insecticides within the rhizosphere. evaluate chapters supply overviews of bioremediation and rhizosphere microbiology.

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Moreover, the only possible role for N 0 was to oxidize caffeic acid: it could not be used as a respiratory terminal electron acceptor due to the presence of acetylene. It is thus suggested that an oxidized derivative of CAF was able to accept the electronsfromAzospirillum metabolism. 5 kPa 0 alone (Figure 4). This suggests that the electron acceptor for ARA was predominantly 0 and secondarily the oxidized derivative of caffeic acid. An unclear point is the actual reoxidizing agent: is N 0 oxidizing CAF directly through the oxidation of another intermediary compound, such as Fe (28).

2 2 Enzymatic Activity of Cells and Cell-free Extracts of A. lipoferum 4T. No spontaneous changes in UV spectra of CAF, 3,4-DHBA, and CAT were ever observed when these compounds were incubated in potassium buffer only (Table HI). No changes in UV-spectra of CAT were observed in any treatment: fresh solution, CAT + 4T cell-free extract, and CAT + 4T cell-free extract + NADPH . During incubation of 4T cells with caffeic acid in potassium phosphate buffer, the absorbance peak of the supernatant shiftedfrom285 nm to 269 nm after 4h of incubation.

Ch003 4 2 4 2 4 4 2 4 4 2 2 2 2 2 2 2 2 5 -2 Culture Conditions. For growth experiments, 5 x 10 ml of NaCl cell suspension were introduced in 150-ml incubation flasks filled with 30 ml of the media (see above). Two oxygen levels were used: atmospheric conditions, i. e. around 21% 0 (21 kPa partial pressure 0 ) in flasks capped with cotton plugs or 5% 0 (5 kPa partial pressure 0 ) in flasks capped with rubber stoppers. To obtain the low 0 partial pressure, flasks filled with media were evacuated threetimesand filled with helium (high grade).

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