By Yvonne A. Barnett, Christopher R. Barnett
Getting older equipment and Protocols offers a few cutting-edge protocols, defined by means of famous specialists of their box, that are getting used to extra our figuring out of the getting older technique. those particular biochemical, actual and molecular biology recommendations are mixed to supply a superb source for researchers and scientists from a wide selection of disciplines. additionally integrated is a presentation of 2 case experiences that target the position of nutritional limit in existence span extension, in addition to how you can identify and make the most of transgenic animals for the elucidation of the molecular facets of getting older. This e-book is a useful resource of cutting-edge study protocols with vast clinical applicability and should curiosity medical researchers, clinicians concerned inIn this paintings, specialists element key biochemical, analytical, and molecular concepts for the research of getting old on the mobile, tissue, organ, and full method degrees.
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For flasks and bottles, the medium should be removed by aspiration or decanting from the side opposite the cell growth surface. For cell culture plates and dishes, the medium should be removed by aspiration from the edge of the growth surface. 3. 25% trypsin solution (4 mL). 4. Remove residual trypsin solution by aspiration from the side opposite the cell growth surface (flasks) or from the edge of the growth surface (plates, dishes, and slides) as appropriate. 5. 25%) to wet the entire cell sheet (2 mL/T-75).
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