Aging Methods and Protocols by Yvonne A. Barnett, Christopher R. Barnett

By Yvonne A. Barnett, Christopher R. Barnett

Getting older equipment and Protocols offers a few cutting-edge protocols, defined by means of famous specialists of their box, that are getting used to extra our figuring out of the getting older technique. those particular biochemical, actual and molecular biology recommendations are mixed to supply a superb source for researchers and scientists from a wide selection of disciplines. additionally integrated is a presentation of 2 case experiences that target the position of nutritional limit in existence span extension, in addition to how you can identify and make the most of transgenic animals for the elucidation of the molecular facets of getting older. This e-book is a useful resource of cutting-edge study protocols with vast clinical applicability and should curiosity medical researchers, clinicians concerned inIn this paintings, specialists element key biochemical, analytical, and molecular concepts for the research of getting old on the mobile, tissue, organ, and full method degrees.

Show description

Read or Download Aging Methods and Protocols PDF

Best geriatrics books

Hazzard's Geriatric Medicine & Gerontology, 6th Edition (Principles of Geriatric Medicine & Gerontology)

The main entire, authoritative advisor to be had at the prognosis and remedy of problems affecting the aged -- up-to-date with a brand new worldwide perspectiveA Doody's center name for 2015! "In addition to serving as a well timed, finished, state of the art textbook of geriatric drugs anchored in technology, evidence-based drugs, and patient-centered perform, the e-book is also meant to fulfill the training wishes of men in geriatric medication.

Introduzione alla genetica forense: Indagini di identificazione personale e di paternità

In quest’opera sono riportate le conoscenze più aggiornate sulle indagini del DNA che sono comunemente utilizzate in step with l’identificazione di autori di reati violenti nelle indagini criminali, according to l’attribuzione dell’identit� a resti umani ed a vittime di disastri di massa, consistent with l. a. ricostruzione di rapporti parentali nelle indagini di paternit� .

Anomia: neuroanatomical and cognitive correlates

Anomia is the lack to entry spoken names for gadgets, customarily linked to the aged or people with mind harm to the left hemisphere. Anomia deals the state of the art assessment of issues of naming, written through said specialists from all over the world, approached from either medical and theoretical viewpoints.

Primary Care Geriatrics: A Case-Based Approach, 5e

Designed for someone concerned with treating geriatric sufferers, this re-creation remains to be the easiest complete resource for scientific strategies for the not easy geriatric inhabitants. within, you will find a wealth of knowledge at the rules of geriatric fundamental care. .. specified, case-based techniques to significant geriatric syndromes.

Extra resources for Aging Methods and Protocols

Example text

For flasks and bottles, the medium should be removed by aspiration or decanting from the side opposite the cell growth surface. For cell culture plates and dishes, the medium should be removed by aspiration from the edge of the growth surface. 3. 25% trypsin solution (4 mL). 4. Remove residual trypsin solution by aspiration from the side opposite the cell growth surface (flasks) or from the edge of the growth surface (plates, dishes, and slides) as appropriate. 5. 25%) to wet the entire cell sheet (2 mL/T-75).

Ponten, J. ), INSERM, Paris, pp. 53–64. 7. Rheinwald, J. G. and Green, H. (1975) Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells. Cell 6, 331–343. 8. Bierman, E. L. (1978) The effects of donor age on the in vitro life span of cultured human arterial smooth-muscle cells. In Vitro 14, 951–955. 9. , and Courtois, Y. (1979) Human lens cells have an in vitro proliferative capacity inversely proportional to the donor age. Exp. Cell Res.

42. Stein, G. , Drullinger, L. , Robetorye, R. , Pereira-Smith, O. , and Smith, J. R. (1991) Senescent cells fail to express cdc2, cycA, and cycB in response to mitogen stimulation. Proc. Natl. Acad. Sci. USA 88, 11,012–11,016. 43. Cristofalo, V. , Doggett, D. , Brooks-Frederich, K. , and Phillips, P. D. (1989) Growth factors as probes of cell aging. Exp. Gerontol. 24, 367–374. 44. , Phillips, P. , and Cristofalo, V. J. (1986) The effect of dexamethasone on epidermal growth factor binding and stimulation of proliferation in young and senescent WI38 cells.

Download PDF sample

Rated 4.58 of 5 – based on 8 votes