By R. W. Fairbrother (Auth.)
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Extra resources for A Text-Book of Medical Bacteriology
Many of the earlier views on the bactericidal action of desiccation appear to require revision. Heat. Heat is the most widely applied bactericidal agent available. I t has already been noted that, on increasing the temperature above t h a t required for optimal growth, reproduction gradually decreases while the destructive action becomes more and more marked. This destructive action continues until all the bacteria in the suspension are destroyed. 56 A TEXT-BOOK OF MEDICAL BACTERIOLOGY The destructive action of heat can be conveniently divided into two classes—dry heat and moist heat.
In nature putrefaction and decay usually occur side by side. All types of albuminous material may be attacked by bacteria. In the laboratory indications of proteolytic activity are usually obtained by the power of the various bacteria to liquefy gelatin, fibrin or coagulated serum, to produce indole or H 2 S, to reduce nitrates to nitrites, and to digest milk and meat. The enzymes responsible for protein-cleavage are termed proteases. The process is usually, in the first stages, a simple hydrolytic cleavage of the complex protein molecule as a result THE BIOLOGY OF BACTERIA 31 of which proteoses, peptones and amino-acids are produced.
The plugs must not be removed and placed on the bench during the seeding of the tube. Such a practice is certain to result in contamination. In seeding fluid media the charged loop should be passed immediately below the surface and rubbed gently against the side of the tube. Vigorous agitation of the loop should be avoided. In seeding solid media the arrangement of the medium must be considered. For a slope culture the loop should be pressed gently but firmly on the surface of the lowest part of the medium and drawn up the surface by passing from side to side.