A Text-Book of Bacteriology by R. W. Fairbrother and Geoffrey A. Taylor (Auth.)

By R. W. Fairbrother and Geoffrey A. Taylor (Auth.)

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C) The second stage is followed by a period at which the viable count remains steady; this is consequently termed the stationary phase. During this period the number of bacteria dying balances the formation of fresh forms. The reason for the marked decrease in the multiplica.. ion of the bacteria has not been deterrnined with certainty, but it appears to depend to some extent on the production of noxious substances during the second phase and to the lack of oxygen in the case of some aerobic organ isms, (d) Tho final stage is one of decline, the number of viable forms gradually decreases until all are dead.

Aerobic and Anaerobic Cultivation. Bacteria vary in their reaction to atrnospheric conditions. Many will grow under both aerobic and anaerobic conditions, but others are either strict aerobes or anaerobes. Thus by cultivating in the presence of, and in the absence of, oxygen it is possible to separate the obligate aerobes from the obligate anaerobes. 5. Selective Media. One of the 1110st frequently applied methods of obtaining pure cultures is by the use of selective media, which usually contain inhibitory substances and indicators.

It frequently happens that material sent for examination contains a number of different bacteria, and the first stage of the examination is obviously the isolation of the individual species. Since the introduction of solid media by Koch in 1881 considerable progress has been made in this direction. Numerous methods have been described, and while some are of doubtful value others have proved highly satisfactory. The important methods will be briefly considered. 1. By Dilution. Before the introduction of solid media the isolation of pure cultures was a matter of considerable difficulty.

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